Bioresources Engineering
MOLECULAR AND MICROBIAL BIOLOGY
Description
Theory
1
Theory/Practice
2
Instructors
Valentina Fernandes Domingues
Contents
CP1. Microbial Growth:
1.1. Influence of internal and external factors on growth dynamics.
1.2. Fed-batch culture: industrial scale processes.
CP2 Inoculum Preparation
2.1 Criteria for the preparation of the inoculum for the fermentation process.
2.2. Inoculum transfer to fermenter and industrial process.
CP3. Environmental Microbiology:
3.1. Water quality from a microbiological point of view.
3.2. Microorganisms and wastewater treatment.
3.3. Microorganisms, quality and sustainable soil management.
3.4. Air microbiology; biological aerosols.
CP4. Microbial genomics and genomics
4.1. Introduction
4.2. Concept of spontaneous mutation vs. induced; mutagenesis; genetic recombination
4.3. Exploration of DNA cloning in biotechnology using different recombination methods
CP5. Industrial Enzymes:
5.1. Properties of the main classes.
5.2. Production.
5.3. Strategies for enzyme immobilization.
Learning Outcomes
PO1- To consolidate and deepen the concepts and principles of microbiology;
PO2- To recognize the importance of the interrelationships between microbial activity and environmental protection and quality;
PO3- Recognize the importance of metabolic diversity of microorganisms as a source of innovation in the production of bioproducts and as a source of wealth creation;
PO4- To characterize the dynamics of microbial growth;
PO5- To understand the steps of preparation of the inoculum for the scale-up of fermentation processes;
PO6- To recognize the importance of the enzymes used in the industry and the strategies for their immobilization;
PO7- To recognize the different applications of molecular biology and its interdisciplinarity with the other sciences;
PO8-To explain the structure and function of nucleic acids and regulation of gene expression;
PO9- Apply basic concepts and fundamentals of methods of manipulation and analysis of nucleic acids.